Anti-NFAT2 antibody [EPR2431(N)(B)] (ab177464)


  • Product name
    Anti-NFAT2 antibody [EPR2431(N)(B)]
    See all NFAT2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2431(N)(B)] to NFAT2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NFAT2 aa 900 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: O95644

  • Positive control
    • Jurkat, Raji and Ramos cell lysates. Raji and Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab177464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


WB 1/1000 - 1/10000. Predicted molecular weight: 101 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
    • Tissue specificity
      Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.
    • Sequence similarities
      Contains 1 RHD (Rel-like) domain.
    • Domain
      Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
      The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
      Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.
    • Post-translational
      Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
    • Information by UniProt
    • Database links
    • Alternative names
      • cytoplasmic 1 antibody
      • MGC138448 antibody
      • NF ATc antibody
      • NF ATc1 antibody
      • NF-ATc antibody
      • NF-ATc1 antibody
      • NF-ATc1.2 antibody
      • NFAC1_HUMAN antibody
      • NFAT 2 antibody
      • NFAT transcription complex cytosolic component antibody
      • NFATC 1 antibody
      • NFATc antibody
      • NFATc1 antibody
      • Nuclear factor of activated T cells cytoplasmic 1 antibody
      • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody
      • Nuclear factor of activated T cells cytosolic component 1 antibody
      • nuclear factor of activated T-cells 'c' antibody
      • Nuclear factor of activated T-cells antibody
      see all


    • Flow cytometric analysis of permeabilized Jurkat cells labeling NFAT2  (red)  with ab177464 at 1/10 dilution, or a rabbit IgG (negative) (green).

    • Immunofluorescence analysis of Raji cells labeling NFAT2 (green) with ab177464 at 1/50 dilution. DAPI nuclear staining (blue).

    • All lanes : Anti-NFAT2 antibody [EPR2431(N)(B)] (ab177464) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 101 kDa


    ab177464 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (Hu pluripotent stem cell derived epicardial like)
    Yes - saponin
    Hu pluripotent stem cell derived epicardial like
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted May 04 2016


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