Anti-NFAT4 (phospho S165) antibody (ab182649)

Overview

  • Product name
    Anti-NFAT4 (phospho S165) antibody
    See all NFAT4 primary antibodies
  • Description
    Rabbit polyclonal to NFAT4 (phospho S165)
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human NFAT4 (phospho S165) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of Serine 165 (S-P-S(p)-P-A) derived from Human NFAT4. NCBI Protein#: NP_004546.1.
    Database link: Q12968

  • Positive control
    • HeLa cell extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    PBS (without Mg2+and Ca2+)
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab182649 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 115 kDa.

Target

  • Function
    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.
  • Tissue specificity
    Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
  • Sequence similarities
    Contains 1 RHD (Rel-like) domain.
  • Domain
    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications
    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links
  • Alternative names
    • C80703 antibody
    • cytoplasmic 3 antibody
    • D8Ertd281e antibody
    • NF AT4 antibody
    • NF ATc3 antibody
    • NF-AT4 antibody
    • NF-ATc3 antibody
    • NFAC3_HUMAN antibody
    • NFATc3 antibody
    • NFATx antibody
    • Nuclear factor of activated T cells, cytoplasmic 3 antibody
    • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 antibody
    • Nuclear factor of activated T-cells antibody
    • T cell transcription factor NFAT4 antibody
    • T-cell transcription factor NFAT4 antibody
    see all

Images

  • All lanes : Anti-NFAT4 (phospho S165) antibody (ab182649) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with antigen-specific peptide


    Predicted band size : 115 kDa

References

ab182649 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab182649.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up