• Product nameAnti-NFAT4 (phospho S165) antibody
    See all NFAT4 primary antibodies
  • Description
    Rabbit polyclonal to NFAT4 (phospho S165)
  • SpecificityDetects endogenous levels of NFAT4 only when phosphorylated at serine 165.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphoryation site of serine 165 (SPSPPA)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab59204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21889978
WB 1/500 - 1/1000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
ELISA 1/5000.


  • FunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.
  • Tissue specificityIsoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
  • Sequence similaritiesContains 1 RHD (Rel-like) domain.
  • DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links
  • Alternative names
    • C80703 antibody
    • cytoplasmic 3 antibody
    • D8Ertd281e antibody
    • NF AT4 antibody
    • NF ATc3 antibody
    • NF-AT4 antibody
    • NF-ATc3 antibody
    • NFAC3_HUMAN antibody
    • NFATc3 antibody
    • NFATx antibody
    • Nuclear factor of activated T cells, cytoplasmic 3 antibody
    • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 antibody
    • Nuclear factor of activated T-cells antibody
    • T cell transcription factor NFAT4 antibody
    • T-cell transcription factor NFAT4 antibody
    see all

Anti-NFAT4 (phospho S165) antibody images

  • All lanes : Anti-NFAT4 (phospho S165) antibody (ab59204) at 1/500 dilution

    Lane 1 : HeLa cells treated with calcium (40nM, 30 mins)
    Lane 2 : HeLa cells treated with calcium (40nM, 30 mins) with immunizing phosphopeptide

    Predicted band size : 116 kDa
    Observed band size : 116 kDa

References for Anti-NFAT4 (phospho S165) antibody (ab59204)

This product has been referenced in:
  • Saygili E  et al. Rate and irregularity of electrical activation during atrial fibrillation affect myocardial NGF expression via different signalling routes. Cell Signal : (2011). WB, ICC/IF ; Rat . Read more (PubMed: 21889978) »
  • Lin Z  et al. miR-23a functions downstream of NFATc3 to regulate cardiac hypertrophy. Proc Natl Acad Sci U S A 106:12103-8 (2009). WB ; Rat . Read more (PubMed: 19574461) »

See all 2 Publications for this product

Product Wall

The cell treatment protocol is as follows:

a. HeLa cells were plated on flasks or dishes containing growth medium (basic medium containing 10% serum).
b. When the cells were at the log phase and 80-90% confluent, the culture me...

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