NFAT5 overexpression 293T lysate (whole cell) (ab94270)

Overview

  • Product nameNFAT5 overexpression 293T lysate (whole cell)
  • Description
    NFAT5 overexpression lysate
  • General notesab94270 is a 293T cell transfected lysate in which Human NFAT5 has been transiently over-expressed using a pCMV-NFAT5 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Plays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes. Similarity: Contains 1 RHD (Rel-like) domain. Tissue specificity: Highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon.

Applications

Our Abpromise guarantee covers the use of ab94270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

NFAT5 overexpression 293T lysate (whole cell) images

  • ab94270 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-NFAT5 antibody - ChIP Grade (ab56997) at 1/500 dilution

    Lane 1 : NFAT5 overexpression 293T lysate (whole cell) (ab94270)
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for NFAT5 overexpression 293T lysate (whole cell) (ab94270)

ab94270 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94270.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"