Overview

  • Product name
    Anti-NFATC2IP antibody
  • Description
    Mouse polyclonal to NFATC2IP
  • Host species
    Mouse
  • Specificity
    In theory, the antibody could interact with isoform1 too since isoform2 is only a truncated version of isoform 1. Only isoform 2 has been tested (NFATC2IP transfected 293T cell lysate).
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cynomolgus monkey
  • Immunogen

    Recombinant fragment corresponding to Human NFATC2IP aa 1-138.
    Sequence:

    MSEPLQSVVDHMATHLGVSPSRILLLFGETELSPTATPRTLKLGVADIID CVVLTSSPEATETSQQLQLRVQGKEKHQTLEVSLSRDSPLKTLMSHYEEA MGLSGRKLSFFFDGTKLSGRELPADLGMESGDLIEVWG

  • Positive control
    • ICC/IF: HeLa cells. WB: NFATC2IP transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab169140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa.

Only isoform 2 has been tested (NFATC2IP transfected 293T cell lysate).

ICC/IF Use a concentration of 10 µg/ml. Antibody purification with Protein A will be needed prior to use.

Target

  • Function
    Plays a role in the inducible expression of cytokine genes in T-cells, especially by increasing NFAT-driven IL-4 production.
  • Sequence similarities
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications
    Methylation at the N-terminus by PRMT1 modulates interaction with the NFAT complex and results in augmented cytokine production.
  • Cellular localization
    Nucleus. Cytoplasm. TRAF1 is associated with a fraction of NFATC2IP in the cytoplasm and prevents its translocation to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 45 kDa NF-AT-interacting protein antibody
    • 45 kDa NFAT-interacting protein antibody
    • cytoplasmic 2-interacting protein antibody
    • ESC2 antibody
    • NF2IP_HUMAN antibody
    • NFAT-interacting protein, 45-KD antibody
    • NFATC2-interacting protein antibody
    • Nfatc2ip antibody
    • NIP45 antibody
    • Nuclear factor of activated T-cells antibody
    • Nuclear factor of activated T-cells, cytoplasmic 2-interacting protein antibody
    • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein antibody
    • RAD60 antibody
    see all

Images

  • Immunofluorescence analysis of permeabilized, paraformaldehyde-fixed HeLa cells, labeling NFATC2IP with ab169140 at 10 µg/ml.
  • All lanes : Anti-NFATC2IP antibody (ab169140) at 1 µg/ml

    Lane 1 : NFATC2IP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa

References

ab169140 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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