Overview

  • Product name
  • Description
    Rabbit polyclonal to NFATC4
  • Specificity
    This antibody detects endogenous levels of total NFATC4 protein.
  • Tested applications
    Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthesized non-phosphopeptide derived from human NFATC4 around the phosphorylation site of serine 676

  • Positive control
    • HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab62613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. (See also PubMed ID 19828810)
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.
  • Tissue specificity
    Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.
  • Sequence similarities
    Contains 1 IPT/TIG domain.
    Contains 1 RHD (Rel-like) domain.
  • Domain
    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications
    Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.
    Ubiquitinated, leading to its degradation by the proteasome and reduced transcriptional activity. Ubiquitination and reduction in transcriptional activity can be further facilitated through GSK3B-dependent phosphorylation. Polyubiquitin linkage is mainly through 'Lys-48'.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic 4 antibody
    • NF ATc4 antibody
    • NF-AT3 antibody
    • NF-ATc4 antibody
    • NFAC4_HUMAN antibody
    • NFAT3 antibody
    • NFATc4 antibody
    • Nuclear factor of activated T cells cytoplasmic 4 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 4 antibody
    • Nuclear factor of activated T-cells antibody
    • T cell transcription factor NFAT3 antibody
    • T-cell transcription factor NFAT3 antibody
    see all

Images

  • Immunofluorescence analysis of HeLa cells, using ab62613 at a 1:500 dilution.

    Left image untreated.

    Right image treated with peptide.

  • Ab62613 staining human normal colon tissue. Staining is localised to subcellular compartment.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:
  • Tian Z  et al. ANGPTL2 activity in cardiac pathologies accelerates heart failure by perturbing cardiac function and energy metabolism. Nat Commun 7:13016 (2016). Read more (PubMed: 27677409) »
  • Hessmann E  et al. NFATc4 Regulates Sox9 Gene Expression in Acinar Cell Plasticity and Pancreatic Cancer Initiation. Stem Cells Int 2016:5272498 (2016). WB, ChIP . Read more (PubMed: 26697077) »

See all 7 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
10% Goat Serum and 4% BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Rat Cell (Isolated Cardiac Fibroblasts)
Specification
Isolated Cardiac Fibroblasts
Permeabilization
Yes - PBS 0.05% Tween 20 - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Aaron West

Verified customer

Submitted Jun 12 2013

Thank you for contacting us. Your replacement with a new lot of ab62613 will ship on order ***.

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made...

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The NFATC4 protein has24 isoforms with a wide range of molecular weights. The predicted molecular weights are approximately8, 20, 36, 47, and there are alsomany isoforms between 77-100 kDa. The full list of known ...

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The western blot conditions will be standard: denatured and reduced samples, block the membrane with either 5% milk or BSA, incubate with antibody diluted 1/500-1/1000 overnight at 4C or one hour at room temperature. We know of one reference which used...

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Thank you for contacting us. The reactivity of the NFATC4 antibody ab62613 was tested by ELISA against the immunogen sequence, which was not phosphorylated at serine 676, and it was also tested for reactivity with the same sequence but phosphorylat...

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