Overview

  • Product nameAnti-NFATC4 antibody
    See all NFATC4 primary antibodies
  • Description
    Rabbit polyclonal to NFATC4
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide: SQRSAQELPQ VEAYSPSACS VRGGEELVLT GSNFLPDSKV VFIERGPDGK , corresponding to amino acids 578-627 of Human NFATC4

  • Positive control
    • Transfected 293T cell lysate and human muscle tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately >87 kDa (predicted molecular weight: 95 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.
  • Tissue specificityHighly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.
  • Sequence similaritiesContains 1 IPT/TIG domain.
    Contains 1 RHD (Rel-like) domain.
  • DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications
    Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.
    Ubiquitinated, leading to its degradation by the proteasome and reduced transcriptional activity. Ubiquitination and reduction in transcriptional activity can be further facilitated through GSK3B-dependent phosphorylation. Polyubiquitin linkage is mainly through 'Lys-48'.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic 4 antibody
    • NF ATc4 antibody
    • NF-AT3 antibody
    • NF-ATc4 antibody
    • NFAC4_HUMAN antibody
    • NFAT3 antibody
    • NFATc4 antibody
    • Nuclear factor of activated T cells cytoplasmic 4 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 4 antibody
    • Nuclear factor of activated T-cells antibody
    • T cell transcription factor NFAT3 antibody
    • T-cell transcription factor NFAT3 antibody
    see all

Anti-NFATC4 antibody images

  • Anti-NFATC4 antibody (ab66781) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 95 kDa
    Observed band size : >87 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 21 kDa,68 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling NFATC4 with ab66781 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

References for Anti-NFATC4 antibody (ab66781)

ab66781 has not yet been referenced specifically in any publications.

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