Overview

  • Product nameAnti-NFATC4 antibody
    See all NFATC4 primary antibodies
  • Description
    Rabbit polyclonal to NFATC4
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NFATC4.

    (Peptide available as ab108242.)

  • Positive control
    • This antibody gave a positive signal in both HeLa and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab93625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 94 kDa).

Target

  • FunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.
  • Tissue specificityHighly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.
  • Sequence similaritiesContains 1 IPT/TIG domain.
    Contains 1 RHD (Rel-like) domain.
  • DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications
    Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.
    Ubiquitinated, leading to its degradation by the proteasome and reduced transcriptional activity. Ubiquitination and reduction in transcriptional activity can be further facilitated through GSK3B-dependent phosphorylation. Polyubiquitin linkage is mainly through 'Lys-48'.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic 4 antibody
    • NF ATc4 antibody
    • NF-AT3 antibody
    • NF-ATc4 antibody
    • NFAC4_HUMAN antibody
    • NFAT3 antibody
    • NFATc4 antibody
    • Nuclear factor of activated T cells cytoplasmic 4 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 4 antibody
    • Nuclear factor of activated T-cells antibody
    • T cell transcription factor NFAT3 antibody
    • T-cell transcription factor NFAT3 antibody
    see all

Anti-NFATC4 antibody images

  • All lanes : Anti-NFATC4 antibody (ab93625) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 94 kDa
    Observed band size : 87 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes
  • ICC/IF image of ab93625 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93625, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-NFATC4 antibody (ab93625)

ab93625 has not yet been referenced specifically in any publications.

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