Overview

  • Product name
  • Description
    Rabbit polyclonal to NFATC4
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chimpanzee, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 300-350 of Human NFATC4 (NP_004545.2).

  • Positive control
    • Human HeLa and 293T cell lines. Mouse NIH3T3 cell line. This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab99431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.
  • Tissue specificity
    Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.
  • Sequence similarities
    Contains 1 IPT/TIG domain.
    Contains 1 RHD (Rel-like) domain.
  • Domain
    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications
    Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.
    Ubiquitinated, leading to its degradation by the proteasome and reduced transcriptional activity. Ubiquitination and reduction in transcriptional activity can be further facilitated through GSK3B-dependent phosphorylation. Polyubiquitin linkage is mainly through 'Lys-48'.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic 4 antibody
    • NF ATc4 antibody
    • NF-AT3 antibody
    • NF-ATc4 antibody
    • NFAC4_HUMAN antibody
    • NFAT3 antibody
    • NFATc4 antibody
    • Nuclear factor of activated T cells cytoplasmic 4 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 4 antibody
    • Nuclear factor of activated T-cells antibody
    • T cell transcription factor NFAT3 antibody
    • T-cell transcription factor NFAT3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NFATC4 with ab99431 at 1/1000 (0.2µg/ml). Detection: DAB.
  • ICC/IF image of ab99431 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99431 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99431 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: NFAT3 antibody (ab99431) at 0.4 µg/ml

    developed using the ECL technique

    Exposure time : 10 seconds

    Predicted band size : 95 kDa

  • All lanes : Anti-NFATC4 antibody (ab99431) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 95 kDa


    Exposure time : 10 seconds

References

This product has been referenced in:
  • Xiao T  et al. Phosphorylation of NFAT3 by CDK3 induces cell transformation and promotes tumor growth in skin cancer. Oncogene 36:2835-2845 (2017). Read more (PubMed: 27893713) »
  • Ding B  et al. Reciprocal autoregulation by NFI occupancy and ETV1 promotes the developmental expression of dendrite-synapse genes in cerebellar granule neurons. Mol Biol Cell 27:1488-99 (2016). WB ; Mouse . Read more (PubMed: 26941328) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab99431.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up