ab41851 gave a positive result in the following Human Whole Cell Lysates: Hela, Jurkat, A431, HEK293, HepG2 and MCF-7.
In addition, ab41851 gave a postive result in the following Tissue Lysates: Human Liver, Mouse Liver, Rat Liver (Data not shown), Rat Brain (Data not shown).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at a concentration of 1 µg/ml.
IHC-Fr: See Abreview for dilution recommendations
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRecognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Sequence similaritiesBelongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain.
ICC/IF image of ab41851 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41851, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Frozen sections) - NFIA antibody (ab41851)This image is courtesy of an abreview submitted by Dr Sophie Pezet
Ab41851 staining NFIA in motorneurons of rat brain tissue sections by IHC-Fr. Sections were PFA fixed and incubated with ab41851, diluted 1/100, for 24 hours at 4°C. Chromeo™ 488 conjugated goat polyclonal to rabbit IgG ab60314, diluted 1/2000, was used as the secondary antibody.