• Product nameAnti-NFIA antibody
    See all NFIA primary antibodies
  • Description
    Rabbit polyclonal to NFIA
  • Tested applicationsSuitable for: WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human NFIA.

    (Peptide available as ab45354.)

  • Positive control
    • ab41851 gave a positive result in the following Human Whole Cell Lysates: Hela, Jurkat, A431, HEK293, HepG2 and MCF-7. In addition, ab41851 gave a postive result in the following Tissue Lysates: Human Liver, Mouse Liver, Rat Liver (Data not shown), Rat Brain (Data not shown).



Our Abpromise guarantee covers the use of ab41851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    IHC-Fr: See Abreview for dilution recommendations
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 56 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRecognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
    • Sequence similaritiesBelongs to the CTF/NF-I family.
      Contains 1 CTF/NF-I DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1110047K16Rik antibody
      • 9430022M17Rik antibody
      • CCAAT Box Binding Transcription Factor antibody
      • CCAAT-box-binding transcription factor antibody
      • CTF antibody
      • FLJ39164 antibody
      • NF I/A antibody
      • NF-I/A antibody
      • NF1-A antibody
      • NFI A antibody
      • NFI L antibody
      • NFI-A antibody
      • NFIA antibody
      • NFIA_HUMAN antibody
      • Nuclear factor 1 A type antibody
      • Nuclear factor 1 A-type antibody
      • Nuclear factor 1/A antibody
      • Nuclear factor I/A antibody
      • TGGCA binding protein antibody
      • TGGCA-binding protein antibody
      see all

    Anti-NFIA antibody images

    • All lanes : Anti-NFIA antibody (ab41851) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 7 : Human liver tissue lysate - total protein (ab29889)
      Lane 8 : Liver (Mouse) Mouse Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 56 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab41851 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41851, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • Ab41851 staining NFIA in motorneurons of rat brain tissue sections by IHC-Fr.  Sections were PFA fixed and incubated with ab41851, diluted 1/100, for 24 hours at 4°C. Chromeo™ 488 conjugated goat polyclonal to rabbit IgG ab60314, diluted 1/2000, was used as the secondary antibody.

      See Abreview

    References for Anti-NFIA antibody (ab41851)

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (brain sections)
    Specification brain sections
    Fixative Paraformaldehyde
    Permeabilization No

    Dr. Sophie Pezet

    Verified customer

    Submitted Jun 30 2008