Anti-NFIB / NF1B2 antibody [NFI5I299] (ab51352)

Overview

  • Product nameAnti-NFIB / NF1B2 antibody [NFI5I299]
    See all NFIB / NF1B2 primary antibodies
  • Description
    Mouse monoclonal [NFI5I299] to NFIB / NF1B2
  • Tested applicationsSuitable for: Dot blot, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • HeLa cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
  • ClonalityMonoclonal
  • Clone numberNFI5I299
  • IsotypeIgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab51352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
IP Use at 20 µg/mg of lysate.
WB 1/100. Detects a band of approximately 49 kDa (predicted molecular weight: 47 kDa).

Target

  • FunctionRecognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • Sequence similaritiesBelongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCAAT Box Binding Transcription Factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CTF antibody
    • HMGIC/NFIB antibody
    • NF-I/B antibody
    • NF1-B antibody
    • NF1B antibody
    • NF1B2 antibody
    • NFI-B antibody
    • NFI-RED antibody
    • Nfib antibody
    • NFIB_HUMAN antibody
    • NFIB2 antibody
    • NFIB3 antibody
    • Nuclear factor 1 B-type antibody
    • Nuclear factor 1/B antibody
    • Nuclear Factor 1B antibody
    • Nuclear Factor I B antibody
    • Nuclear factor I/B antibody
    • TGGCA Binding Protein antibody
    • TGGCA-binding protein antibody
    • TRANSCRIPTION FACTOR NFIB antibody
    see all

Anti-NFIB / NF1B2 antibody [NFI5I299] images

  • ab51252 used at 10µg/500µg RIPA lysate of HeLa cells. Western blot labelled with ab51352 at 1/100 dilution followed by TrueBlot anti Mouse IgG at 1/1000 dilution. Developed using the ECL technique.

References for Anti-NFIB / NF1B2 antibody [NFI5I299] (ab51352)

ab51352 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E17 Mouse brain slices, free floating)
Specification E17 Mouse brain slices, free floating
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - 0.2% Triton X
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Randal Moldrich

Verified customer

Submitted Jul 08 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (E17 Mouse brain slices)
Specification E17 Mouse brain slices
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X
Blocking step Goat serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Randal Moldrich

Verified customer

Submitted Jul 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"