ab54403 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 36 kDa for the recombinant fragment (predicted molecular weight of the full length protein: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain.