Overview

  • Product nameAnti-NFIC antibody
    See all NFIC primary antibodies
  • Description
    Rabbit polyclonal to NFIC
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 324-373 (SPFNSPSPQD SPRLSSFTQH HRPVIAVHSG IARSPHPSSA LHFPTTSILP) of human NFIC (NP_995315).

  • Positive control
    • MCF7 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRecognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • Sequence similaritiesBelongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110019L22Rik antibody
    • 1500041O16Rik antibody
    • AA589446 antibody
    • AI746521 antibody
    • CAAT box transcription factor antibody
    • CCAAT binding transcription factor antibody
    • CCAAT box binding transcription factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CNFI C antibody
    • CTF antibody
    • CTF5 antibody
    • MGC137374 antibody
    • MGC20153 antibody
    • NF I antibody
    • NF I/C antibody
    • NF-I/C antibody
    • NF1 C antibody
    • NF1-C antibody
    • NF1C antibody
    • NFI antibody
    • NFI-C antibody
    • NFI/C antibody
    • NFIC antibody
    • NFIC_HUMAN antibody
    • Nuclear factor 1 antibody
    • Nuclear factor 1 C type antibody
    • Nuclear factor 1 C-type antibody
    • Nuclear factor 1/C antibody
    • Nuclear factor I/C (CCAAT binding transcription factor) antibody
    • Nuclear factor I/C antibody
    • TGGCA binding protein antibody
    • TGGCA-binding protein antibody
    • Transcription factor NFIC antibody
    see all

Anti-NFIC antibody images

  • Anti-NFIC antibody (ab86570) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • ab86570 staining NFIC in human adipocytes by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-NFIC antibody (ab86570)

ab86570 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse Mandibles and Teeth)
Specification Mouse Mandibles and Teeth
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization Yes - 0.1% Triton in PBS
Blocking step Background Terminator as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 27°C
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Submitted Mar 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"