Overview

  • Product nameAnti-NFIL3 antibody
    See all NFIL3 primary antibodies
  • Description
    Rabbit polyclonal to NFIL3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human NFIL3.

  • Positive control
    • This antibody gave a positive signal in the following Human whole cell lysates: HeLa; K562.

Properties

Applications

Our Abpromise guarantee covers the use of ab88526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).

Target

  • FunctionActs as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death.
  • Tissue specificityExpressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.
  • Sequence similaritiesBelongs to the bZIP family. NFIL3 subfamily.
    Contains 1 bZIP domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E4 promoter binding protein 4 antibody
    • E4 promoter-binding protein 4 antibody
    • E4BP 4 antibody
    • E4BP4 antibody
    • E4BP4 protein antibody
    • IL3BP 1 antibody
    • IL3BP1 antibody
    • Interleukin 3 binding protein 1 antibody
    • Interleukin 3 promoter transcriptional activator antibody
    • Interleukin-3 promoter transcriptional activator antibody
    • Interleukin-3-binding protein 1 antibody
    • NFIL 3 antibody
    • NFIL 3A antibody
    • NFIL3 antibody
    • NFIL3_HUMAN antibody
    • NFIL3A antibody
    • Nuclear factor interleukin 3 regulated antibody
    • Nuclear factor interleukin-3-regulated protein antibody
    • Transcriptional activator NF IL3A antibody
    • Transcriptional activator NF-IL3A antibody
    see all

Anti-NFIL3 antibody images

  • All lanes : Anti-NFIL3 antibody (ab88526) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-NFIL3 antibody (ab88526)

ab88526 has not yet been referenced specifically in any publications.

Product Wall

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