Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab101341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl 1/1000. PubMed: 23776487
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.

Target

  • Function
    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • Sequence similarities
    Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCAAT box binding transcription factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CTF antibody
    • NF-I/X antibody
    • NF1-X antibody
    • NF1A antibody
    • NF1X antibody
    • NFI X antibody
    • NFI-X antibody
    • NFI/X antibody
    • NFIX antibody
    • NFIX_HUMAN antibody
    • Nuclear factor 1 X type antibody
    • Nuclear factor 1 X-type antibody
    • Nuclear factor 1/X antibody
    • Nuclear factor I/X antibody
    • TGGCA binding protein antibody
    • TGGCA-binding protein antibody
    see all

Images

  • Immunohistochemistry (Free Floating) analysis of adult mouse (wild type and NFix+/-) coronal brain tissue. Sections were blocked for 2 hours with serum before incubation overnight with ab101341 (1/1000 in blocking buffer). Sections were then incubated with a biotin-conjugated goat anti-rabbit IgG (1/1,000) secondary antibody for 1 hour. The reaction was visualised by incubating the sections in avidin-biotin complex for 1 hour, followed by a nickel-DAB solution, and was terminated by washing multiple times in phosphate buffered saline when a dark precipitate had formed. Sections were then mounted onto slides, dehydrated and imaged.

    In adult wild-type mice (G), NFIX was most strongly expressed by cells in the SGZ of the dentate gyrus (arrowheads in G’). The same expression pattern was observed in Nfix+/− mice, although the level of NFIX expression was much lower than in wild-type mice at all ages. G’ and H’ are magnified views of the boxed regions in G and H respectively.

  • Anti-NFIX antibody (ab101341) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 55 kDa
    10% SDS PAGE

References

This product has been referenced in:
  • Harris L  et al. Heterozygosity for nuclear factor one x affects hippocampal-dependent behaviour in mice. PLoS One 8:e65478 (2013). IHC-FrFl ; Mouse . Read more (PubMed: 23776487) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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