The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at a concentration of 10 µg/ml. Protein A affinity purification required prior to immunofluorescence application.
WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRecognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Sequence similaritiesBelongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain.