Overview

  • Product name
  • Description
    Mouse polyclonal to NFIX
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-441 of Human NFIX

  • Positive control
    • HeLa cells, Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml. Protein A affinity purification required prior to immunofluorescence application.
    WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).
    Detection Pair


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
    • Sequence similarities
      Belongs to the CTF/NF-I family.
      Contains 1 CTF/NF-I DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCAAT box binding transcription factor antibody
      • CCAAT-box-binding transcription factor antibody
      • CTF antibody
      • NF-I/X antibody
      • NF1-X antibody
      • NF1A antibody
      • NF1X antibody
      • NFI X antibody
      • NFI-X antibody
      • NFI/X antibody
      • NFIX antibody
      • NFIX_HUMAN antibody
      • Nuclear factor 1 X type antibody
      • Nuclear factor 1 X-type antibody
      • Nuclear factor 1/X antibody
      • Nuclear factor I/X antibody
      • TGGCA binding protein antibody
      • TGGCA-binding protein antibody
      see all

    Images

    • Immunofluorescence of purified ab67169 on HeLa cells. Antibody concentration 10 µg/ml.
    • All lanes : Anti-NFIX antibody (ab67169) at 1/500 dilution

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : non Transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 55 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    References

    ab67169 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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