Anti-NFkB Inducing Kinase NIK antibody (ab22042)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab22042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).

Target

  • FunctionLymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
  • Tissue specificityWeakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
    Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP3K14 antibody
    • FTDCR1B antibody
    • HS antibody
    • HsNIK antibody
    • M3K14_HUMAN antibody
    • Map3k14 antibody
    • Mitogen activated protein kinase kinase kinase 14 antibody
    • Mitogen-activated protein kinase kinase kinase 14 antibody
    • NF kappa beta inducing kinase antibody
    • NF-kappa-beta-inducing kinase antibody
    • NIK antibody
    • Serine/threonine-protein kinase NIK antibody
    see all

Anti-NFkB Inducing Kinase NIK antibody images

  • Lane 1 : Anti-NFkB Inducing Kinase NIK antibody (ab22042) at 3 µg/ml (in untransfected 293 cells )
    Lane 2 : Anti-NFkB Inducing Kinase NIK antibody (ab22042) at 3 µg/ml (in transfected 293 cells)

    Lane 1 : As above
    Lane 2 : As above


    Performed under reducing conditions.

    Predicted band size : 105 kDa
    Observed band size : 105 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NFkB Inducing Kinase NIK antibody (ab22042)

ab22042 has not yet been referenced specifically in any publications.

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