Anti-NFkB Inducing Kinase NIK antibody (ab37712)

Overview

  • Product nameAnti-NFkB Inducing Kinase NIK antibody
    See all NFkB Inducing Kinase NIK primary antibodies
  • Description
    Rabbit polyclonal to NFkB Inducing Kinase NIK
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide: IFIAGSKQYS QSESLDQIPN NVAHATEGKM ARVCWKGKRR SKARKKRKKK SSKSLAHAGV ALAKPLPRTP EQESCTIPVQ EDESPLGAPY VRNTPQFTKP L conjugated to KLH, corresponding to amino acids 100-200 of Human NFkB Inducing Kinase NIK

  • Positive control
    • 293 cell lysate. Rat testis tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab37712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 104 kDa.
IHC-P 1/50 - 1/100.

Target

  • FunctionLymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
  • Tissue specificityWeakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
    Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP3K14 antibody
    • FTDCR1B antibody
    • HS antibody
    • HsNIK antibody
    • M3K14_HUMAN antibody
    • Map3k14 antibody
    • Mitogen activated protein kinase kinase kinase 14 antibody
    • Mitogen-activated protein kinase kinase kinase 14 antibody
    • NF kappa beta inducing kinase antibody
    • NF-kappa-beta-inducing kinase antibody
    • NIK antibody
    • Serine/threonine-protein kinase NIK antibody
    see all

Anti-NFkB Inducing Kinase NIK antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling NFkB Inducing Kinase NIK with ab37712 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab37712) at 1/1000 dilution

    Lane 1 : 293 whole cell lysate
    Lane 2 : rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 104 kDa
    Observed band size : 104 kDa
    Additional bands at : 37 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

References for Anti-NFkB Inducing Kinase NIK antibody (ab37712)

This product has been referenced in:

See 1 Publication for this product

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I've confirmed that ab37712 was tested with non-transfected 293 lysate, however ab19144 was actually...

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