Anti-NFkB Inducing Kinase NIK antibody (ab7204)

Overview

  • Product nameAnti-NFkB Inducing Kinase NIK antibody
    See all NFkB Inducing Kinase NIK primary antibodies
  • Description
    Rabbit polyclonal to NFkB Inducing Kinase NIK
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human NFkB Inducing Kinase NIK aa 795-947.
    Database link: Q99558

Properties

Applications

Our Abpromise guarantee covers the use of ab7204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 23301098
WB 1/2000.
IP 1/500.

Target

  • FunctionLymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
  • Tissue specificityWeakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
    Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP3K14 antibody
    • FTDCR1B antibody
    • HS antibody
    • HsNIK antibody
    • M3K14_HUMAN antibody
    • Map3k14 antibody
    • Mitogen activated protein kinase kinase kinase 14 antibody
    • Mitogen-activated protein kinase kinase kinase 14 antibody
    • NF kappa beta inducing kinase antibody
    • NF-kappa-beta-inducing kinase antibody
    • NIK antibody
    • Serine/threonine-protein kinase NIK antibody
    see all

Anti-NFkB Inducing Kinase NIK antibody images

  • All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab7204)

    Lane 1 : macrophages lysate
    Lane 2 : NIK transfected cell lysate
  • ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-NFkB Inducing Kinase NIK antibody (ab7204)

This product has been referenced in:
  • Jung JU  et al. NIK/MAP3K14 Regulates Mitochondrial Dynamics and Trafficking to Promote Cell Invasion. Curr Biol N/A:N/A (2016). ICC/IF ; Human . Read more (PubMed: 27889261) »
  • Döppler H  et al. Downregulation of TRAF2 mediates NIK-induced pancreatic cancer cell proliferation and tumorigenicity. PLoS One 8:e53676 (2013). WB, IHC-P ; Human . Read more (PubMed: 23301098) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (colon)
Specification colon
Treatment overexpression
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Liwei Xie

Verified customer

Submitted Apr 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 20 µg
Specification HEK293
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 16 2012

Thank you for your secondary. Ab7092 - Goat polyclonal to Rat IgG H&L Pre-Adsorbed - is a secondary antibody and is not a positive control for ab7204. Ab7204 was characterized for application in Western blotting using NIK transfected cell lysate wh...

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Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please le...

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We haven't tested endogenous NIK but one of our customers has got this to reliably work for detecting endogenous NIK.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"