Anti-NFkB p100 (phospho S869) antibody (ab31475)


  • Product nameAnti-NFkB p100 (phospho S869) antibody
    See all NFkB p100 primary antibodies
  • Description
    Rabbit polyclonal to NFkB p100 (phospho S869)
  • SpecificityAntibody detects endogenous levels of NFkB p100 / p52 protein only when phosphorylated at Serine 869. This antibody detects sites on p100 only. Sometimes there is a 52kDa band on WB; this is not p52. There is a cleavage site on the NFKB2 (Swiss-Prot No.: Q00653). The cleavage site is between 454 and 455 (cleavage when cotranslationally processed). The antibody can interact with the C-half sometimes. The MW. of C-half is near 52Kd.
  • Tested applicationsSuitable for: WB, IHC-P, IP, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human NFkB p100 (phospho S869).
    Database link: Q00653

  • Positive control
    • WB: breast carcinoma tissue, extracts of Hela cells, MOLT cells and MDA-MB-435 cells treated with TNF alpha. IHC-P: breast carcinoma tissue.



Our Abpromise guarantee covers the use of ab31475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/10000.
    IHC-P: 1/50 - 1/100.
    IP: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Detects bands of approximately 67 and 105 kDa (predicted molecular weights: 54 and 97 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceNF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • CVID10 antibody
      • DNA binding factor KBF2 antibody
      • H2TF1 antibody
      • Lymphocyte translocation chromosome 10 protein antibody
      • LYT 10 antibody
      • NF kB2 antibody
      • NFKB p52/p100 subunit antibody
      • Nuclear factor Kappa B subunit 2 antibody
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) antibody
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody
      • Oncogene Lyt 10 antibody
      • p100 antibody
      • Transcription factor NFKB2 antibody
      see all

    Anti-NFkB p100 (phospho S869) antibody images

    • Ab31475, at a dilution of 1/50-1/100, staining NFkB p100 / p52 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
      Left image: Untreated.
      Right image: Antibody pre incubated with synthesized phosphopeptide.

    References for Anti-NFkB p100 (phospho S869) antibody (ab31475)

    ab31475 has not yet been referenced specifically in any publications.

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