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Our Abpromise guarantee covers the use of ab7971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||1/400. Predicted molecular weight: 50 kDa.Can be blocked with NFkB p105 / p50 peptide (ab8006). PubMed: 16395278|
|ChIP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|EMSA||Use at an assay dependent concentration. PubMed: 22553204|
|ICC/IF||Use at an assay dependent concentration.|
ICC/IF image of ab7971 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7971, 5µg/ml) overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Gating Strategy: Isolate cell population from plot of SSC-A / FSA-A
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