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Synthetic peptide derived from the region aa330 to aa433 of the Human NFkB p50 subunit.
Our Abpromise guarantee covers the use of ab7971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|WB||1/400. Predicted molecular weight: 50 kDa.Can be blocked with NFkB p105 / p50 peptide (ab8006). PubMed: 16395278|
|ChIP||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent concentration.|
|EMSA||Use at an assay dependent concentration. PubMed: 22553204|
|ICC/IF||Use at an assay dependent concentration.|
ICC/IF image of ab7971 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7971, 5µg/ml) overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Gating Strategy: Isolate cell population from plot of SSC-A / FSA-A
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