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Synthetic peptide derived from the region aa330 to aa433 of the Human NFkB p50 subunit.
Members of the rel/NFkappaB family of transcription factors are involved in the regulation of cellular responses, such as growth, development, and the inflammatory response. They share a structural motif known as the rel homology region (RHR), the C-terminal one third of which mediates protein dimerization. Complexes of p50 (NF-kB1) or p52 (NF-kB2) are generated through the processing of p105 and p100 precursors, respectively. These are usually associated with members of the Rel family (p65, c-Rel, Rel B). The homo- and heterodimer formed through combinations of NF-kB/Rel proteins bind distinct kB sites to regulate the transcription of different genes.
Our Abpromise guarantee covers the use of ab7971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|WB||1/400. Predicted molecular weight: 50 kDa.Can be blocked with NFkB p105 / p50 peptide (ab8006). PubMed: 16395278|
|ChIP||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent concentration.|
|EMSA||Use at an assay dependent concentration. PubMed: 22553204|
|ICC/IF||Use at an assay dependent concentration.|
Gating Strategy: Isolate cell population from plot of SSC-A / FSA-A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"