Anti-NFKBIL1 antibody - C-terminal (ab170749)

Overview

  • Product name
    Anti-NFKBIL1 antibody - C-terminal
    See all NFKBIL1 primary antibodies
  • Description
    Rabbit polyclonal to NFKBIL1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NFKBIL1 aa 334-364 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9UBC1

  • Positive control
    • Human brain tissue; MDA-MB435 cell lysate; MDA-MB435 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 43 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function
    May be a negative regulator of NF-kappa-B activation.
  • Tissue specificity
    Detected in different cell types including monocytes, T-cells, B-cells and hepatocytes.
  • Involvement in disease
    Defects in NFKBIL1 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
  • Sequence similarities
    Contains 2 ANK repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • I-kappa-B-like protein antibody
    • IkappaBL antibody
    • IKBL antibody
    • IKBL1_HUMAN antibody
    • Inhibitor of kappa B-like protein antibody
    • LST1 antibody
    • NF-kappa-B inhibitor-like protein 1 antibody
    • NFKBIL antibody
    • Nfkbil1 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 antibody
    see all

Images

  • Immunofluorescent analysis of MDA-MB435 cells labeling NFKBIL1 with ab170749 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling NFKBIL1 with ab170749 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Anti-NFKBIL1 antibody - C-terminal (ab170749) at 1/100 dilution + MDA-MB435 cell lysate at 35 µg

    Predicted band size: 43 kDa

References

ab170749 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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