• Product nameAnti-NFKBIL2 antibody
    See all NFKBIL2 primary antibodies
  • Description
    Rabbit polyclonal to NFKBIL2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Leaf frog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human NFKBIL2.

  • Positive control
    • This antibody gave a positive signal in HeLa whole cell lysate and HeLa nuclear lysate.



Our Abpromise guarantee covers the use of ab101898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200. Use paraformaldehyde fixed cells, permeabilized with 0.5% Triton X-100/PBS.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 151 kDa).


  • FunctionComponent of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold.
  • Tissue specificityExpressed in heart, skeletal muscle and tracheal epithelial cells.
  • Sequence similaritiesBelongs to the Tonsoku family.
    Contains 3 ANK repeats.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 8 TPR repeats.
  • DomainThe ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L.
  • Cellular localizationCytoplasm. Nucleus. Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B related protein antibody
    • I-kappa-B-related protein antibody
    • IkappaBR antibody
    • IKBR antibody
    • Inhibitor of kappa B related protein antibody
    • Inhibitor of kappa B-related protein antibody
    • MMS22L-interacting protein antibody
    • MMS22L-interacting protein, NFBIL2/TONSL antibody
    • NF kappa B inhibitor like protein 2 antibody
    • NF-kappa-B inhibitor-like protein 2 antibody
    • NFBIL2 antibody
    • NFBIL2/TONSL antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor like protein 2 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2 antibody
    • Protein IkappaBR antibody
    • Tonsl antibody
    • TONSL_HUMAN antibody
    • Tonsoku-like protein antibody
    • tonsoku-like, DNA repair protein antibody
    see all

Anti-NFKBIL2 antibody images

  • All lanes : Anti-NFKBIL2 antibody (ab101898) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 151 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 43 kDa,67 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ab101898 (1/200) staining NFKBIL2 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

References for Anti-NFKBIL2 antibody (ab101898)

ab101898 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 15 2011