This antibody gave a positive signal in HeLa whole cell lysate and HeLa nuclear lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. Use paraformaldehyde fixed cells, permeabilized with 0.5% Triton X-100/PBS.
Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 151 kDa).
FunctionComponent of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold.
Tissue specificityExpressed in heart, skeletal muscle and tracheal epithelial cells.
Sequence similaritiesBelongs to the Tonsoku family. Contains 3 ANK repeats. Contains 7 LRR (leucine-rich) repeats. Contains 8 TPR repeats.
DomainThe ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L.
Cellular localizationCytoplasm. Nucleus. Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks.
Immunocytochemistry/ Immunofluorescence - Anti-NFKBIL2 antibody (ab101898)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab101898 (1/200) staining NFKBIL2 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.