Purification notesPurified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 50 kDa.
Use at an assay dependent dilution.
FunctionCatalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor.
Tissue specificityPredominantly expressed in heart and skeletal muscle. Also found in brain, liver and pancreas.
Sequence similaritiesBelongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.
Cellular localizationCytoplasm. Nucleus and Mitochondrion.