Overview

  • Product nameAnti-NFS1 antibody
    See all NFS1 primary antibodies
  • Description
    Rabbit polyclonal to NFS1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide: GIIDLKELEA AIQPDTSLVS VMTVNNEIGV KQPIAEIGRI CSSRKVYFHT, corresponding to internal sequence amino acids 182-231 of Human NFS1

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3125 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

Target

  • FunctionCatalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor.
  • Tissue specificityPredominantly expressed in heart and skeletal muscle. Also found in brain, liver and pancreas.
  • Sequence similaritiesBelongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.
  • Cellular localizationCytoplasm. Nucleus and Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cysteine desulfurase antibody
    • Cysteine desulfurase, mitochondrial antibody
    • HUSSY 08 antibody
    • IscS antibody
    • mitochondrial antibody
    • NFS1 antibody
    • NFS1 nitrogen fixation 1 antibody
    • NFS1 nitrogen fixation 1 homolog (S. cerevisiae) antibody
    • NFS1_HUMAN antibody
    • NIFS antibody
    • nitrogen fixation 1 (S. cerevisiae, homolog) antibody
    • nitrogen fixation gene 1 antibody
    • nitrogen-fixing bacteria S, homolog of antibody
    • nitrogen-fixing bacteria S-like protein antibody
    • OTTHUMP00000030799 antibody
    see all

Anti-NFS1 antibody images

  • All lanes : Anti-NFS1 antibody (ab66272) at 0.31 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : markers
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 25 kDa,30 kDa,>70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NFS1 antibody (ab66272)

ab66272 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"