The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3125 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1:312500.
FunctionCatalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor.
Tissue specificityPredominantly expressed in heart and skeletal muscle. Also found in brain, liver and pancreas.
Sequence similaritiesBelongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.
Cellular localizationCytoplasm. Nucleus and Mitochondrion.