Overview

  • Product nameAnti-NFX1 antibody
    See all NFX1 primary antibodies
  • Description
    Rabbit polyclonal to NFX1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 1071-1120 (TLTGVLEREM QARPPPPIPH HRHQSDKNPG SSNLQKITKE PIIDYFDVQD) of Human NFX1 (NP_002495).

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 124 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceNFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. It may play a role in the inflammatory response, regulating its duration by limiting the period in which class II MHC molecules are induced by IFN gamma. The RING type zinc finger domain interacts with an ubiquitin conjugating enzyme (E2) and facilitates ubiquitination.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • MGC20369 antibody
    • NFX 1 antibody
    • NFX1 antibody
    • NFX2 antibody
    • Nuclear transcription factor X box binding 1 antibody
    • Nuclear transcription factor X box binding protein 1 antibody
    • Transcriptional repressor NF X1 antibody
    see all

Anti-NFX1 antibody images

  • Anti-NFX1 antibody (ab83833) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 124 kDa
    Observed band size : 124 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration 6-18%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling NFX1 with ab83833 at 5µg/ml.

References for Anti-NFX1 antibody (ab83833)

ab83833 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"