Overview

  • Product name
  • Description
    Rabbit polyclonal to NFX1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide from within a region of N terminal amino acids 1-50 (AEAPPVSGTF KFNTDAAEFI PQEKKNSGLN CGTQRRLDSN RIGRRNYSSP) of Human NFX1 (NP_667344)

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 124 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. It may play a role in the inflammatory response, regulating its duration by limiting the period in which class II MHC molecules are induced by IFN gamma. The RING type zinc finger domain interacts with an ubiquitin conjugating enzyme (E2) and facilitates ubiquitination.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • MGC20369 antibody
    • NFX 1 antibody
    • NFX1 antibody
    • NFX2 antibody
    • Nuclear transcription factor X box binding 1 antibody
    • Nuclear transcription factor X box binding protein 1 antibody
    • Transcriptional repressor NF X1 antibody
    see all

Images

  • Anti-NFX1 antibody (ab86207) at 1 µg/ml (5% skim milk/ PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 124 kDa

References

ab86207 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab86207.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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