Overview

  • Product nameAnti-NFXL1 antibody
  • Description
    Rabbit polyclonal to NFXL1
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 100-150 of Human NFXL1 (NP_694540.3)

  • Positive control
    • HeLa cell lysate
  • General notesAntibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99.91% Tris citrate/phosphate
    Note: pH: 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab114848 was affinity purified using an epitope specific to NFXL1 immobilized on a solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab114848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • Sequence similaritiesBelongs to the NFX1 family.
      Contains 10 NF-X1-type zinc fingers.
      Contains 1 RING-type zinc finger.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • hOZFP antibody
      • NF-X1-type zinc finger protein NFXL1 antibody
      • NFXL1 antibody
      • NFXL1_HUMAN antibody
      • Nuclear transcription factor antibody
      • Ovarian zinc finger protein antibody
      • Up-regulated in colon cancer 5 antibody
      • URCC5 antibody
      • X-box binding-like 1 antibody
      see all

    Anti-NFXL1 antibody images

    • ab114848 at 6ug/mg of lysate detecting NFXL1 by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
      Detection: chemiluminescence. Exposure time: 30 seconds.

    References for Anti-NFXL1 antibody (ab114848)

    ab114848 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Sample Human Cell (MCF10A cell line)
    Specification MCF10A cell line
    Permeabilization Yes - 0.1% TX-100, 10min
    Fixative Paraformaldehyde
    Username

    Miguel Sanchez

    Verified customer

    Submitted Mar 27 2014

    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing (4-20% gradient HEPES-glycine gel)
    Sample Human Cell lysate - whole cell (MCF10A cell line)
    Specification MCF10A cell line
    Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Miguel Sanchez

    Verified customer

    Submitted Mar 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"