Overview

  • Product name
    Anti-NFXL1 antibody
  • Description
    Rabbit polyclonal to NFXL1
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 100-150 of Human NFXL1 (NP_694540.3)

  • Positive control
    • HeLa cell lysate
  • General notes
    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99.91% Tris citrate/phosphate
    Note: pH: 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab114848 was affinity purified using an epitope specific to NFXL1 immobilized on a solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab114848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Sequence similarities
      Belongs to the NFX1 family.
      Contains 10 NF-X1-type zinc fingers.
      Contains 1 RING-type zinc finger.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • hOZFP antibody
      • NF-X1-type zinc finger protein NFXL1 antibody
      • NFXL1 antibody
      • NFXL1_HUMAN antibody
      • Nuclear transcription factor antibody
      • Ovarian zinc finger protein antibody
      • Up-regulated in colon cancer 5 antibody
      • URCC5 antibody
      • X-box binding-like 1 antibody
      see all

    Images

    • ab114848 at 6ug/mg of lysate detecting NFXL1 by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
      Detection: chemiluminescence. Exposure time: 30 seconds.

    References

    ab114848 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Sample
    Human Cell (MCF10A cell line)
    Specification
    MCF10A cell line
    Permeabilization
    Yes - 0.1% TX-100, 10min
    Fixative
    Paraformaldehyde
    Username

    Miguel Sanchez

    Verified customer

    Submitted Mar 27 2014

    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (4-20% gradient HEPES-glycine gel)
    Sample
    Human Cell lysate - whole cell (MCF10A cell line)
    Specification
    MCF10A cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Miguel Sanchez

    Verified customer

    Submitted Mar 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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