Overview

  • Product name
  • Description
    Rabbit polyclonal to NFYA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 347 of Human NFYA.

  • Positive control
    • A431, HeLa, HepG2, NIH 3T3, JC and BCL1 whole cell lysates; HeLa cells; D54 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • Sequence similarities
    Belongs to the NFYA/HAP2 subunit family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAAT box DNA binding protein subunit A antibody
    • CAAT box DNA-binding protein subunit A antibody
    • CBF A antibody
    • CBF B antibody
    • CBFA antibody
    • CBFB antibody
    • CCAAT binding transcription factor subunit B antibody
    • FLJ11236 antibody
    • HAP 2 antibody
    • HAP2 antibody
    • HAP2 CCAAT binding protein antibody
    • NF YA antibody
    • NF-YA antibody
    • NFY protein chain A antibody
    • NFYA antibody
    • NFYA_HUMAN antibody
    • NUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES antibody
    • Nuclear transcription factor Y alpha antibody
    • Nuclear transcription factor Y antibody
    • Nuclear transcription factor Y subunit A antibody
    • Nuclear transcription factor Y subunit alpha antibody
    • Sez10 antibody
    • Transcription factor NF Y A subunit antibody
    see all

Images

  • All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    10% SDS PAGE
  • All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human D54 xenograft labeling NFYA with ab154554 at 1/500 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cell labeling NFYA with ab154554 at 1/500 dilution. The image in the lower panel was costained with Hoechst 33342.

References

ab154554 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154554.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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