Overview

  • Product nameAnti-NFYA antibody
    See all NFYA primary antibodies
  • Description
    Rabbit polyclonal to NFYA
  • Specificityab23471 reacts with Nuclear Transcription Factor Y, a isoform 1 (NFYA).
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within a synthetic peptide: LHESRHRHAM ARKRGEGGRF FSPKEKDSPH MQDPNQADEE AMTQIIRVS, corresponding to C terminal amino acids 299-348 of Human NFYA

  • Positive control
    • Jurkat cell lysate and human liver.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 - 8 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • Sequence similaritiesBelongs to the NFYA/HAP2 subunit family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAAT box DNA binding protein subunit A antibody
    • CAAT box DNA-binding protein subunit A antibody
    • CBF A antibody
    • CBF B antibody
    • CBFA antibody
    • CBFB antibody
    • CCAAT binding transcription factor subunit B antibody
    • FLJ11236 antibody
    • HAP 2 antibody
    • HAP2 antibody
    • HAP2 CCAAT binding protein antibody
    • NF YA antibody
    • NF-YA antibody
    • NFY protein chain A antibody
    • NFYA antibody
    • NFYA_HUMAN antibody
    • NUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES antibody
    • Nuclear transcription factor Y alpha antibody
    • Nuclear transcription factor Y antibody
    • Nuclear transcription factor Y subunit A antibody
    • Nuclear transcription factor Y subunit alpha antibody
    • Sez10 antibody
    • Transcription factor NF Y A subunit antibody
    see all

Anti-NFYA antibody images

  • Anti-NFYA antibody (ab23471) at 2 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Cells with positive label: Hepatocyte (indicated with arrows). Antibody concentration 4-8ug/ml. Magnification 400X.

References for Anti-NFYA antibody (ab23471)

ab23471 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Entamoeba invadens Cell lysate - nuclear (Nuclear extract)
Specification Nuclear extract
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dipak Manna

Verified customer

Submitted Nov 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"