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Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 16-46 of Human NFYB (NP_006157.1).
Our Abpromise guarantee covers the use of ab123384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 23 kDa.|
|ICC/IF||1/10 - 1/50.|
Immunofluorescence of HeLa cells labelling NFYB with ab123384. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab123384 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFYB immunoreactivity is localized to the nucleus.
ab123384 has not yet been referenced specifically in any publications.