Immunofluorescence of HeLa cells labelling NFYB with ab123384. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab123384 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFYB immunoreactivity is localized to the nucleus.
Western blot - Anti-NFYB antibody (ab123384)
Anti-NFYB antibody (ab123384) at 1/100 dilution + A549 cell line lysates at 35 µg