• Product nameAnti-NFYC antibody
    See all NFYC primary antibodies
  • Description
    Rabbit polyclonal to NFYC
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Ferret, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 325 and 375 of Human NFYC (CAI16533.1).

  • Positive control
    • Whole cell lysate from HeLa cells and 293T cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab86237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • Sequence similaritiesBelongs to the NFYC/HAP5 subunit family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAAT box DNA binding protein subunit C antibody
    • CAAT box DNA-binding protein subunit C antibody
    • CBF C antibody
    • CBFC antibody
    • CCAAT binding factor subunit C antibody
    • CCAAT transcription binding factor subunit gamma antibody
    • DKFZp667G242 antibody
    • FLJ45775 antibody
    • H1TF2A antibody
    • HAP5 antibody
    • histone H1 transcription factor large subunit 2A antibody
    • HSM antibody
    • NF-YC antibody
    • NFYC antibody
    • NFYC_HUMAN antibody
    • Nuclear transcription factor Y gamma antibody
    • Nuclear transcription factor Y subunit C antibody
    • Nuclear transcription factor Y subunit gamma antibody
    • OTTHUMP00000009207 antibody
    • OTTHUMP00000009208 antibody
    • OTTHUMP00000009209 antibody
    • OTTHUMP00000009210 antibody
    • OTTHUMP00000009211 antibody
    • OTTHUMP00000009212 antibody
    • OTTHUMP00000009213 antibody
    • OTTHUMP00000009215 antibody
    • OTTHUMP00000009217 antibody
    • OTTHUMP00000231028 antibody
    • Transactivator HSM 1/2 antibody
    • transactivator HSM-1 antibody
    • Transactivator HSM-1/2 antibody
    see all

Anti-NFYC antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NFYC with ab86237 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NFYC antibody (ab86237) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Exposure time : 10 seconds
  • Detection of NFYC in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86237 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.

References for Anti-NFYC antibody (ab86237)

ab86237 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab86237.
Please use the links above to contact us or submit feedback about this product.