• Product nameAnti-NFYC antibody
    See all NFYC primary antibodies
  • Description
    Rabbit polyclonal to NFYC
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Ferret, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 325 and 375 of Human NFYC (CAI16533.1).

  • Positive control
    • Whole cell lysate from HeLa cells and 293T cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab86237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • Sequence similaritiesBelongs to the NFYC/HAP5 subunit family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAAT box DNA binding protein subunit C antibody
    • CAAT box DNA-binding protein subunit C antibody
    • CBF C antibody
    • CBFC antibody
    • CCAAT binding factor subunit C antibody
    • CCAAT transcription binding factor subunit gamma antibody
    • DKFZp667G242 antibody
    • FLJ45775 antibody
    • H1TF2A antibody
    • HAP5 antibody
    • histone H1 transcription factor large subunit 2A antibody
    • HSM antibody
    • NF-YC antibody
    • NFYC antibody
    • NFYC_HUMAN antibody
    • Nuclear transcription factor Y gamma antibody
    • Nuclear transcription factor Y subunit C antibody
    • Nuclear transcription factor Y subunit gamma antibody
    • OTTHUMP00000009207 antibody
    • OTTHUMP00000009208 antibody
    • OTTHUMP00000009209 antibody
    • OTTHUMP00000009210 antibody
    • OTTHUMP00000009211 antibody
    • OTTHUMP00000009212 antibody
    • OTTHUMP00000009213 antibody
    • OTTHUMP00000009215 antibody
    • OTTHUMP00000009217 antibody
    • OTTHUMP00000231028 antibody
    • Transactivator HSM 1/2 antibody
    • transactivator HSM-1 antibody
    • Transactivator HSM-1/2 antibody
    see all

Anti-NFYC antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NFYC with ab86237 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NFYC antibody (ab86237) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Exposure time : 10 seconds
  • Detection of NFYC in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86237 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.

References for Anti-NFYC antibody (ab86237)

ab86237 has not yet been referenced specifically in any publications.

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