Overview

  • Product nameAnti-NG2 antibody [9.2.27]
    See all NG2 primary antibodies
  • Description
    Mouse monoclonal [9.2.27] to NG2
  • SpecificityRecognizes mature NG2 core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa (Bumoi et al., 1984).
  • Tested applicationsSuitable for: Electron Microscopy, Blocking, IP, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin SepharoseTM (Morgan et al., 1981).

  • Positive control
    • Human melanoma, glioma and proliferating brain endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab78284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
Blocking
IP
Flow Cyt
ICC/IF
  • Application notesBL: Use at an assay dependent dilution. Has been used for functional block of melanoma cell spreading, glioma cell proliferation and tumor growth in nude mice.
    Immunoelectron microscopy: Use at an assay dependent dilution.
    Flow Cyt: Use 1-2µg for 106 cells.
    Immunofluorescence microscopy of cells in culture: Use at an assay dependent dilution.
    IP: Use 1µg for 106 cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
    • Tissue specificityDetected only in malignant melanoma cells.
    • Sequence similaritiesContains 15 CSPG (NG2) repeats.
      Contains 2 laminin G-like domains.
    • Post-translational
      modifications
      O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
      Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
    • Cellular localizationApical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4732461B14Rik antibody
      • AN2 antibody
      • AN2 proteoglycan antibody
      • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
      • Chondroitin sulfate proteoglycan 4 antibody
      • Chondroitin sulfate proteoglycan NG2 antibody
      • Cspg4 antibody
      • Cspg4 chondroitin sulfate proteoglycan 4 antibody
      • CSPG4_HUMAN antibody
      • HMW-MAA antibody
      • HSN tumor-specific antigen antibody
      • Kiaa4232 antibody
      • MCSP antibody
      • MCSPG antibody
      • MEL-CSPG antibody
      • Melanoma chondroitin sulfate proteoglycan antibody
      • Melanoma-associated chondroitin sulfate proteoglycan antibody
      • MELCSPG antibody
      • MSK16 antibody
      • NG2 antibody
      see all

    References for Anti-NG2 antibody [9.2.27] (ab78284)

    This product has been referenced in:
    • Chen SY  et al. Isolation and characterization of mesenchymal progenitor cells from human orbital adipose tissue. Invest Ophthalmol Vis Sci 55:4842-52 (2014). IF ; Human . Read more (PubMed: 24994870) »
    • Schrappe M  et al. Long-term growth suppression of human glioma xenografts by chemoimmunoconjugates of 4-desacetylvinblastine-3-carboxyhydrazide and monoclonal antibody 9.2.27. Cancer Res 52:3838-44 (1992). ICC/IF, Blocking ; Human . Read more (PubMed: 1617657) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your inquiry.

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    Merci de nous avoir contactés.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"