• Product name
  • Description
    Rabbit polyclonal to NG2
  • Host species
  • Tested applications
    Suitable for: IHC-FoFr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1300 - 1400 of Human NG2.

    (Peptide available as ab96112.)

  • Positive control
    • This antibody gave a positive signal in Human Skin Tissue Lysate - adult normal tissue



Our Abpromise guarantee covers the use of ab83178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/100.
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 251 kDa).


  • Function
    Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
  • Tissue specificity
    Detected only in malignant melanoma cells.
  • Sequence similarities
    Contains 15 CSPG (NG2) repeats.
    Contains 2 laminin G-like domains.
  • Post-translational
    O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
    Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
  • Cellular localization
    Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4732461B14Rik antibody
    • AN2 antibody
    • AN2 proteoglycan antibody
    • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
    • Chondroitin sulfate proteoglycan 4 antibody
    • Chondroitin sulfate proteoglycan NG2 antibody
    • Cspg4 antibody
    • Cspg4 chondroitin sulfate proteoglycan 4 antibody
    • CSPG4_HUMAN antibody
    • HMW-MAA antibody
    • HSN tumor-specific antigen antibody
    • Kiaa4232 antibody
    • MCSP antibody
    • MCSPG antibody
    • MEL-CSPG antibody
    • Melanoma chondroitin sulfate proteoglycan antibody
    • Melanoma-associated chondroitin sulfate proteoglycan antibody
    • MELCSPG antibody
    • MSK16 antibody
    • NG2 antibody
    see all


  • IHC-FoFr image of NG2 staining on sections of rat cortex post injury using ab83178 (1:100). The brain was perfusion fixed using 4% PFA and the sections were permeabilized using 0.1% TritonX in 0.1% PBS. The sections were then blocked using 10% donkey serum for 1hour at 24°C. ab83178 was diluted 1:100 and incubated with the sections for 24 hours at 4°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

  • ICC/IF image of ICC/IF staining on miced glia culture cells using ab83178 (1:200). The mixed glia culture was fixed with PFA and the cells were then blocked with 10% donkey serum for 1 hour at 24°C. The cells were then incubated with ab83178 was diluted by 1:200 using 0.3% TritonX with 0.1% PBS and 10% donkey serum. The cells were then incubated with donkey polyclonal to antirabbit conjuaged to Alexa 488 (1:1000). DAPI was used to stain the nucleus.

    See Abreview

  • Anti-NG2 antibody (ab83178) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 251 kDa
    Observed band size: 300 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 190 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes

    Human Chondroitin sulfate proteoglycan 4 precursor (NG2) contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.


This product has been referenced in:
  • Ye X  et al. Altered ratios of pro- and anti-angiogenic VEGF-A variants and pericyte expression of DLL4 disrupt vascular maturation in infantile haemangioma. J Pathol 239:139-51 (2016). IHC-P ; Human . Read more (PubMed: 26957058) »
  • Raha AA  et al. The systemic iron-regulatory proteins hepcidin and ferroportin are reduced in the brain in Alzheimer's disease. Acta Neuropathol Commun 1:55 (2013). Read more (PubMed: 24252754) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Pig Cell (brain Pericytes)
Yes - 0,1% TritonX
brain Pericytes
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: RT°C

Mrs. Verena Ledwig

Verified customer

Submitted Mar 09 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Rat Tissue sections (Rat Brain post injury)
Rat Brain post injury
Yes - 0.1% TritonX in 0.1% PBS

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 27 2013

Merci de nous avoir contactés. Je suis désolé d'apprendre que vous avez des difficultés à obtenir de bons résultats avec ab83178.

Notre politique est d'offrir tout d'abord la meilleure assistance techn...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Rat Cell (Glial culture)
Glial culture
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jul 09 2012


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