Overview

  • Product nameAnti-NG2 antibodySee all NG2 primary antibodies ...
  • Description
    Mouse monoclonal to NG2
  • Tested applicationsIHC-P, WB, IP, ELISA, Flow Cyt, ICC/IF, IHC-FrFl more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Cell membrane preparation from Human malignant melanoma SK-MEL-28.

  • Positive control
    • Human melanoma tissue. SK-MEL-28 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab83508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use a concentration of 2 - 5 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 251 kDa.
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
Flow Cyt 1/10 - 1/100.
ICC/IF Use at an assay dependent concentration. PubMed: 22701703
IHC-FrFl Use at an assay dependent concentration.

Target

  • FunctionProteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
  • Tissue specificityDetected only in malignant melanoma cells.
  • Sequence similaritiesContains 15 CSPG (NG2) repeats.
    Contains 2 laminin G-like domains.
  • Post-translational
    modifications
    O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
    Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
  • Cellular localizationApical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
  • Target information above from: UniProt accession Q6UVK1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 4732461B14Rik antibody
    • AN2 antibody
    • AN2 proteoglycan antibody
    • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
    • Chondroitin sulfate proteoglycan 4 antibody
    • Chondroitin sulfate proteoglycan NG2 antibody
    • CSPG4 antibody
    • Cspg4 chondroitin sulfate proteoglycan 4 antibody
    • CSPG4_HUMAN antibody
    • HMW-MAA antibody
    • HSN tumor-specific antigen antibody
    • Kiaa4232 antibody
    • MCSP antibody
    • MCSPG antibody
    • MEL-CSPG antibody
    • Melanoma chondroitin sulfate proteoglycan antibody
    • Melanoma-associated chondroitin sulfate proteoglycan antibody
    • MELCSPG antibody
    • MSK16 antibody
    see all

Anti-NG2 antibody images

  • ab83508 at a 1/100 dilution staining NG2 in human melanoma tissue.
  • 5 x 106 SK-MEL-28 cells were incubated with 5µg of ab83508, followed by APC-GtxMs IgG incubation.
  • Immunofluorescence analysis of Human adult blood endothelial progenitor cells (left) and umbilical artery smooth muscle cells (right).

    NG2 was stained using ab83508 at 1/200 dilution. Nuclei were stained with DAPI (blue).

References for Anti-NG2 antibody (ab83508)

This product has been referenced in:
  • Hsu NC  et al. High chondroitin sulfate proteoglycan 4 expression correlates with poor outcome in patients with breast cancer. Biochem Biophys Res Commun 441:514-8 (2013). IHC-P ; Human . Read more (PubMed: 24177010) »
  • Keats E & Khan ZA Unique responses of stem cell-derived vascular endothelial and mesenchymal cells to high levels of glucose. PLoS One 7:e38752 (2012). ICC/IF ; Human . Read more (PubMed: 22701703) »

See all 3 Publications for this product

Product Wall

The stabilizer contains BSA, proclin (˜0.05%), and another component which does not affect the cell culture. However, if you want to be safe you can sterile-filtrate the antibodies before applying this antibody to the cell culture.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Melanoma)
Specification Melanoma
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 10 2013

Application Flow Cytometry
Sample Human Cell (Melanoma)
Specification Melanoma
Preparation Cell harvesting/tissue preparation method: Cell scraper. Pulse/rinse with PBS.
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy R660 positive cells (APC+). Gate determined by secondary antibody alone.
Username

Dr. Simon Jones

Verified customer

Submitted May 14 2013

Thank you for contacting us.

The ab83508 reacts with human and mouse samples. It was used for IHC on free floating sections (IHC-FrFl) ( Abreview http://www.abcam.com/index.html?datasheet=83508&tab=abreviews&intabreviewid=26915 ) and I...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - triton 0.5%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 28 2012

Thank you once againfor your enquiry and for your patience.

I can confirm that we have mouse as a tested and guaranteed species from the IHC-FrFl Abreview published on the datasheet for ab83508.

I am sorry the originator does not ...

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DISCOUNT CODE: 100%ABR-EBVXU


Expiration date: 26/11/2012

I am very pleased to hear you would like to accept our offer and testab83508 in IHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To ...

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Thank you very much for your interest in ab83508.

To our knowledge,ab83508 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buyab83508 now, test it inIHC-Fr and submit feedback to us in the form o...

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Thank you for your enquiry regarding ab83508.

This antibody has been tested and characterized for Immunohistochemistry on formalin-fixed paraffin-embedded tissue sections (IHC-P) and on Free Floating samples (IHC-FrFl). We have not specifical...

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Thank you for your inquiry.

I have to confirm that we do not offer custom conjugation for antibodies.

I can suggest to conjugate the antibody yourself with our EasyLink kit for Biotin:

ab102865

http://www.abcam.c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"