Anti-NG2 antibody [LHM 2] (ab20156)
Key features and details
- Mouse monoclonal [LHM 2] to NG2
- Suitable for: IHC-Fr, IP, IHC-FoFr, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NG2 antibody [LHM 2]
See all NG2 primary antibodies -
Description
Mouse monoclonal [LHM 2] to NG2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IP, IHC-FoFr, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human NG2. a 375P cell crude extract
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General notes
The LHM2 antibody is a very good marker for melanoma (>90% stained). LHM2 also stains basal cell carcinomas and subpopulation of basal keratinocytes. LHM2 can be used as a diagnostic panel for immunopathology of malignant melanoma and potential candidates for immunoimaging and immunotherapy of disseminated melanoma. The originators of this antibody report that the MCSP is a cell surface marker specific for epidermal stem cells (J. Invest. Dermatol. in press). Potential applications of the LHM2 antibody include use for separation/isolation of epidermal stem cells, detection of stem cells in skin biopsies or epithelial cell cultures and optimisation of culture conditions to retain epidermal stem cell population in in vitro cultures.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
LHM 2 -
Myeloma
NS0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 21970342
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 21970342 |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
Target
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Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. -
Tissue specificity
Detected only in malignant melanoma cells. -
Sequence similarities
Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains. -
Post-translational
modificationsO-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. -
Cellular localization
Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes. - Information by UniProt
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Database links
- Entrez Gene: 1464 Human
- Omim: 601172 Human
- SwissProt: Q6UVK1 Human
- Unigene: 513044 Human
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Alternative names
- 4732461B14Rik antibody
- AN2 antibody
- AN2 proteoglycan antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab20156 has been referenced in 13 publications.
- Bang-Christensen SR et al. Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein. Cells 8:N/A (2019). PubMed: 31466397
- Kim JM et al. Perivascular Progenitor Cells Derived From Human Embryonic Stem Cells Exhibit Functional Characteristics of Pericytes and Improve the Retinal Vasculature in a Rodent Model of Diabetic Retinopathy. Stem Cells Transl Med 5:1268-76 (2016). Human . PubMed: 27388242
- Loibl M et al. Direct cell-cell contact between mesenchymal stem cells and endothelial progenitor cells induces a pericyte-like phenotype in vitro. Biomed Res Int 2014:395781 (2014). ICC/IF . PubMed: 24563864
- Somaiah N et al. The relationship between homologous recombination repair and the sensitivity of human epidermis to the size of daily doses over a 5-week course of breast radiotherapy. Clin Cancer Res 18:5479-88 (2012). IHC-P ; Human . PubMed: 22855580
- Hyldahl RD et al. Activation of nuclear factor-?B following muscle eccentric contractions in humans is localized primarily to skeletal muscle-residing pericytes. FASEB J 25:2956-66 (2011). IHC-P ; Human . PubMed: 21602448