Overview

  • Product name
  • Description
    Rabbit polyclonal to NGDN
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 61-209 of Human NGDN (Q8NEJ9).

  • Positive control
    • NGDN transfected HEK293T lysate, Human skin tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

  • Function
    Involved in the translational repression of cytoplasmic polyadenylation element (CPE)-containing mRNAs.
  • Sequence similarities
    Belongs to the SAS10 family.
  • Cellular localization
    Nucleus. Cytoplasm. Cell projection > axon. Cell projection > dendrite. Cell projection > filopodium. Detected in axons, dendrites and filopodia. Colocalized with EIF4E in neurites.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf120 antibody
    • EIF4E-binding protein antibody
    • Neuroguidin antibody
    • NGDN antibody
    • NGDN_HUMAN antibody
    see all

Images

  • Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus, nucleoli & mitochondria.
  • All lanes : Anti-NGDN antibody (ab121124) at 1/250 dilution

    Lane 1 : vector only transfected HEK293T lysate
    Lane 2 : NGDN-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

    Developed using the ECL technique10-20% SDS PAGE
  • ab121124 at 1/350 dilution staining NGDN in Paraffin-emebedded Human skin tissue by Immunohistochemistry. Note: strong nuclear positivity in epidermal cells.

References

ab121124 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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