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Native mouse salivary gland NGF purified by modification of the method of Mobley et al (1976). Antigen purity was greater than 95% by PAGE.
Our Abpromise guarantee covers the use of ab6198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|IHC-Fr||1/400 - 1/1000.|
|Neutralising||1/2 - 1/10.|
|WB||1/400 - 1/1000.|
|IHC-P||1/400 - 1/1000.|
ab6198 at 1/1000 staining perfusion fixed rat brain tissue sections by IHC-Fr. The animals were pre-perfused with Tris buffer pH 10, followed by 4% paraformadehyde and 15% of a saturated solution of picric acid. The brains were post-fixed in the same fixative overnight, cryoprotected in 20% sucrose for 24 hours, frozen and cut with a cryostat. Free floating immunostaining was performed. An Alexa-Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.
The image shows the staining obtained with this antibody using direct fluorescence in rat cortical neurons, with two different magnifications of acquisition (X10 and X20).