Overview

  • Product name
  • Description
    Rabbit polyclonal to NGF
  • Tested applications
    Suitable for: Neutralising, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hNGFb (human Nerve Growth Factor beta).

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising
WB
ELISA
  • Application notes
    ELISA: Use at a concentration of 0.5 µg/ml, allows the detection of 0.2 - 0.4 ng/well of recombinant hNGFb.

    WB: Use at a concentration of 0.1 - 0.2 µg/ml, detection limit for recombinant hNGFb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    Neut: To yield one-half maximal inhibition [ND50] of the biological activity of NGF (1 ng/ml), a concentration of 0.05-0.08 µg/ml of this antibody is required.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
    • Involvement in disease
      Neuropathy, hereditary sensory and autonomic, 5
    • Sequence similarities
      Belongs to the NGF-beta family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta nerve growth factor antibody
      • Beta NGF antibody
      • Beta-nerve growth factor antibody
      • Beta-NGF antibody
      • HSAN5 antibody
      • MGC161426 antibody
      • MGC161428 antibody
      • Nerve growth factor (beta polypeptide) antibody
      • Nerve growth factor antibody
      • Nerve growth factor beta antibody
      • Nerve growth factor beta polypeptide antibody
      • Nerve growth factor beta subunit antibody
      • NGF antibody
      • NGF_HUMAN antibody
      • NGFB antibody
      • NID67 antibody
      see all

    References

    This product has been referenced in:
    • Ferrari MP  et al. Safety and pharmacokinetics of escalating doses of human recombinant nerve growth factor eye drops in a double-masked, randomized clinical trial. BioDrugs 28:275-83 (2014). Read more (PubMed: 24327173) »

    See 1 Publication for this product

    Customer reviews and Q&As


    The source of the recombinant immunogen used to produce ab9795 is E. coli.

    Thank you for contacting us. Please find attached the CoC form. At -20C the antibodies are viable for 10 years. At 4C these are viable for 1 year. Please be advised that Abcam guarantee is applicable on products for 6 months on...

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    Thank you for contacting us. We can certainly provide the Certificate of Compliance. Could you send me the lot number of antibody used? Antibodies are stable for years when stored at proper temperature e.g. at -20C the antibodies are via...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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