Anti-NGF antibody [AS21] (ab16161)

Overview

  • Product nameAnti-NGF antibody [AS21]
    See all NGF primary antibodies
  • Description
    Mouse monoclonal [AS21] to NGF
  • SpecificityRecognises 2.5S, 7S and bNGF.
  • Tested applicationsSuitable for: Sandwich ELISA, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human).

  • EpitopeAmino acids 46-62 of mature human NGF.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab16161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.
ELISA Use a concentration of 10 µg/ml. Can be paired for ELISA with Mouse monoclonal [AS18] to NGF (Biotin) (ab16160). (For coating in a two site ELISA with biotin conjugated NGF antibody for mouse NGF). Two site ELISA.
Neutralising Use at an assay dependent dilution. This antibody has been used to neutralize > 90% human NGF bioactivity on PC12 cells at a 200 fold molar excess.

Target

  • FunctionNerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
  • Involvement in diseaseNeuropathy, hereditary sensory and autonomic, 5
  • Sequence similaritiesBelongs to the NGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta nerve growth factor antibody
    • Beta NGF antibody
    • Beta-nerve growth factor antibody
    • Beta-NGF antibody
    • HSAN5 antibody
    • MGC161426 antibody
    • MGC161428 antibody
    • Nerve growth factor (beta polypeptide) antibody
    • Nerve growth factor antibody
    • Nerve growth factor beta antibody
    • Nerve growth factor beta polypeptide antibody
    • Nerve growth factor beta subunit antibody
    • NGF antibody
    • NGF_HUMAN antibody
    • NGFB antibody
    • NID67 antibody
    see all

References for Anti-NGF antibody [AS21] (ab16161)

ab16161 has not yet been referenced specifically in any publications.

Product Wall


We obtained better results in ELISA with TBS compared to PBS, so I definitely recommend using TBS if possible. The protocol details are as follows, if you need more details than this please let me know and I will contact the lab for them:

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Thank you for your enquiry and patience. This antibody has been used to neutralize more than 90% human NGF bioactivity on PC12 cells at a 200 fold molar excess. Unfortunately, I cannot obtain additional details regarding this. If you have any additiona...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"