Overview

  • Product name
  • Description
    Rabbit polyclonal to NHEDC1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MHTTESKNEH LEDENFQTST TPQSLIDPNN TAHEETKTVL SDTEEIKPQT) of Human NHEDC1 (NP_001094344).

  • Positive control
    • Human fetal brain cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Tissue specificity
    Expressed only in the testis.
  • Sequence similarities
    Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700094G20Rik antibody
    • 4933424B12Rik antibody
    • 4933425K02Rik antibody
    • AV258602 antibody
    • MGC131641 antibody
    • Na(+)/H(+) exchanger-like domain-containing protein 1 antibody
    • NHDC1_HUMAN antibody
    • NHE domain-containing protein 1 antibody
    • Nhedc1 antibody
    • Sodium/hydrogen exchanger-like domain-containing protein 1 antibody
    see all

Images

  • Anti-NHEDC1 antibody (ab106124) at 0.5 µg/ml + Human fetal brain cell lysate at 10 µg

    Predicted band size : 56 kDa

References

This product has been referenced in:
  • Kumar PL & James PF Identification and characterization of methylation-dependent/independent DNA regulatory elements in the human SLC9B1 gene. Gene 561:235-48 (2015). Read more (PubMed: 25701605) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for keeping us updated on your results. I am sorry to hear that the secondary antibody is gives non-specific bands. I'm assuming that you ran a blot without the primary antibody, and that is how you know the extra bands are from the secondary...

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Thank you for your reply. Regarding the sample buffer, we used the following:  0.05M Tris-HCl, pH6.8, 0.1M DTT, 2%SDS, 10% glycerol, 0.1% bromophenol blue Although slightly, different, your buffer would be fine as well. Samples were then heated...

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Thank you for contacting Abcam regarding ab106124. Please find the WB protocol used to validate this antibody below: - Prepare 12% gel - Load 10ug human fetal brain cell lysate - Transfer protein to PVDF membrane (we used Bio-Rad Trans-Blot Ce...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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