The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
FunctionElectroneutral exchange of protons for Na(+) and Li(+) across the inner mitochondrial membrane. Contributes to the organellar volume homeostasis. Required for osteoclast differentiation and bone resorption activity.
Tissue specificityDetected in red blood cells (at protein level).
Sequence similaritiesBelongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.