The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 21300888
1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
RelevanceNHLH1 and NHLH2 are two basic domain helix-loop-helix (bHLH) proteins expressed predominantly in the developing nervous system and also in some cell lines derived from tumors with neural or neuroendocrine features such as neuroblastoma, PNET, and small cell lung cancer.
All lanes : Anti-NHLH1 + NHLH2 antibody (ab72601) at 1/500 dilution
Lane 1 : extracts from Jurkat cells Lane 2 : extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 15 kDa Observed band size : 15 kDa
References for Anti-NHLH1 + NHLH2 antibody (ab72601)
This product has been referenced in:
Klisch TJ et al. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A108:3288-93 (2011).
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