Overview

  • Product name
  • Description
    Rabbit polyclonal to NHP2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to amino acids 81-150 of Human NHP2

  • Positive control
    • Human colon tissue. RT-4, U-251 MG, Human plasma and liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab150918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
  • Tissue specificity
    Expressed in brain, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Also expressed at lower levels in the liver.
  • Involvement in disease
    Dyskeratosis congenita, autosomal recessive, 2
  • Sequence similarities
    Belongs to the ribosomal protein L7Ae family.
  • Developmental stage
    Transcript peaks at G1/S transition.
  • Cellular localization
    Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKCB2 antibody
    • FLJ20479 antibody
    • H/ACA ribonucleoprotein complex subunit 2 antibody
    • NHP2 antibody
    • NHP2 like protein antibody
    • NHP2 ribonucleoprotein antibody
    • NHP2 ribonucleoprotein homolog (yeast) 1 antibody
    • NHP2 ribonucleoprotein homolog antibody
    • NHP2, S. cerevisiae, homolog of antibody
    • NHP2_HUMAN antibody
    • NHP2P antibody
    • NOLA2 antibody
    • Nucleolar protein family A member 2 (H/ACA small nucleolar RNPs) antibody
    • Nucleolar protein family A member 2 antibody
    • snoRNP protein NHP2 antibody
    see all

Images

  • All lanes : Anti-NHP2 antibody (ab150918) at 1/100 dilution

    Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
    Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)


    Predicted band size : 17 kDa
  • All lanes : Anti-NHP2 antibody (ab150918) at 1/100 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Liver lysate
    Lane 5 : Tonsil lysate


    Predicted band size : 17 kDa
  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon tissue labelling NHP2 with ab150918 at 1/50 dilution.

References

ab150918 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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