Recombinant
RabMAb

Anti-NHP2 antibody [EPR12410] (ab172481)

Overview

  • Product name
    Anti-NHP2 antibody [EPR12410]
    See all NHP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12410] to NHP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NHP2 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NX24

  • Positive control
    • K562, Jurkat, Caco 2 and SH-SY5Y cell lysate; Human pancreas and spleen tissues; Caco 2 and K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
    • Tissue specificity
      Expressed in brain, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Also expressed at lower levels in the liver.
    • Involvement in disease
      Dyskeratosis congenita, autosomal recessive, 2
    • Sequence similarities
      Belongs to the ribosomal protein L7Ae family.
    • Developmental stage
      Transcript peaks at G1/S transition.
    • Cellular localization
      Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKCB2 antibody
      • FLJ20479 antibody
      • H/ACA ribonucleoprotein complex subunit 2 antibody
      • NHP2 antibody
      • NHP2 like protein antibody
      • NHP2 ribonucleoprotein antibody
      • NHP2 ribonucleoprotein homolog (yeast) 1 antibody
      • NHP2 ribonucleoprotein homolog antibody
      • NHP2, S. cerevisiae, homolog of antibody
      • NHP2_HUMAN antibody
      • NHP2P antibody
      • NOLA2 antibody
      • Nucleolar protein family A member 2 (H/ACA small nucleolar RNPs) antibody
      • Nucleolar protein family A member 2 antibody
      • snoRNP protein NHP2 antibody
      see all

    Images

    • All lanes : Anti-NHP2 antibody [EPR12410] (ab172481) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Caco 2 cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling NHP2 with ab172481 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling NHP2 with ab172481 at 1/50 dilution.

    • Immunofluorescent staining of Caco 2 cells labeling NHP2 with ab172481 at 1/50 dilution.

    • Flow cytometric analysis of permeabilized K562 cells labeling NHP2 with ab172481 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).

    References

    ab172481 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human embryonic stem cell, 293T, HeLa)
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Loading amount
    50 µg
    Specification
    human embryonic stem cell, 293T, HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Jianlong Wang

    Verified customer

    Submitted Mar 19 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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