Anti-NHP2L1 antibody (ab95958)
Key features and details
- Rabbit polyclonal to NHP2L1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-NHP2L1 antibody
See all NHP2L1 primary antibodies -
Description
Rabbit polyclonal to NHP2L1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Zebrafish -
Immunogen
Recombinant fragment corresponding to Human NHP2L1 aa 1-98.
Database link: P55769 -
Positive control
- WB: HeLa and HepG2 whole cell lysates ICC/IF: Hela cell IHC-P: Cal27 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 14 kDa.
|
IHC-P |
1/100 - 1/250.
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ICC/IF | (1) |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 14 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/200. |
Target
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Relevance
Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri snRNP. It binds to the 5' stem loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. -
Cellular localization
Nucleus › nucleolus. Note: Concentrated in the dense fibrillar component of the nucleolus. -
Database links
- Entrez Gene: 4809 Human
- Entrez Gene: 321114 Zebrafish
- Omim: 601304 Human
- SwissProt: P55769 Human
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Alternative names
- [U4/U6.U5] tri snRNP antibody
- 15.5 kD RNA binding protein antibody
- High mobility group like nuclear protein 2 homolog 1 antibody
see all
Images
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All lanes : Anti-NHP2L1 antibody (ab95958) at 1/1000 dilution
Lane 1 : Hela whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa
12% SDS PAGE -
ab95958, at a 1/200 dilution, staining NHP2L1 in paraformaldehyde fixed HeLa cell by Immunofluorescence analysis.
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ab95958, at a 1/100 dilution, staining NHP2L1 in paraffin embedded Cal27 xenograft by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab95958 has been referenced in 5 publications.
- Koedoot E et al. Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention. J Exp Clin Cancer Res 40:82 (2021). PubMed: 33648524
- Diouf B et al. Identification of small molecules that mitigate vincristine-induced neurotoxicity while sensitizing leukemia cells to vincristine. Clin Transl Sci 14:1490-1504 (2021). PubMed: 33742760
- Falaleeva M et al. Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing. Proc Natl Acad Sci U S A 113:E1625-34 (2016). PubMed: 26957605
- Tremblay N et al. Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response. PLoS Pathog 12:e1005772 (2016). WB . PubMed: 27454487
- Sundaramoorthy S et al. Functional genomics identifies a requirement of pre-mRNA splicing factors for sister chromatid cohesion. EMBO J N/A:N/A (2014). WB . PubMed: 25257310