Recombinant
RabMAb

Anti-NHP2L1 antibody [EPR11671] (ab181982)

Overview

  • Product name
    Anti-NHP2L1 antibody [EPR11671]
    See all NHP2L1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11671] to NHP2L1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NHP2L1 aa 1-100. The exact sequence is proprietary.
    Database link: P55769

  • Positive control
    • HepG2, HeLa, Jurkat and MCF-7 cells. Human lung carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/1000.
ICC/IF 1/500.
IP 1/40.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri snRNP. It binds to the 5' stem loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene.
  • Cellular localization
    Nucleus › nucleolus. Note: Concentrated in the dense fibrillar component of the nucleolus.
  • Database links
  • Alternative names
    • [U4/U6.U5] tri snRNP antibody
    • 15.5 kD RNA binding protein antibody
    • High mobility group like nuclear protein 2 homolog 1 antibody
    • hSNU13 antibody
    • NHP2 non histone chromosome protein 2 like 1 (S. cerevisiae) antibody
    • NHP2L 1 antibody
    • NHP2L1 antibody
    • NHPX antibody
    • OTK27 antibody
    • SNU13 antibody
    see all

Images

  • All lanes : Anti-NHP2L1 antibody [EPR11671] (ab181982) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : MCF-7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

  • Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labeling NHP2L1 with ab181982 at 1/1000 dilution followed by secondary Ready to use HRP Polymer for Rabbit IgG and hematoxylin counterstain.

  • Immunofluorescence analysis of HeLa cells labeling NHP2L1 with ab181982 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor® 555) (1/200 dilution) and counter stained with Dapi.

  • Immunoprecipitation analysis of MCF-7 cells labeling NHP2L1 using ab181982 at 1/40 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

  • Flow cytometry analysis of MCF-7 cells labeling NHP2L1 using ab181982 at 1/50 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.

References

ab181982 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HES2 human embryonic stem cell, 293T, HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
HES2 human embryonic stem cell, 293T, HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Jianlong Wang

Verified customer

Submitted Dec 22 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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