• Product nameAnti-Nicastrin antibody
    See all Nicastrin primary antibodies
  • Description
    Rabbit polyclonal to Nicastrin
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic 18 amino acid peptide from near the center of human Nicastrin.

  • Positive control
    • Mouse brain tissue lysate.



Our Abpromise guarantee covers the use of ab45425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).


  • FunctionEssential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the nicastrin family.
  • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anterior pharynx defective 2 antibody
    • APH 2 antibody
    • APH2 antibody
    • ATAG1874 antibody
    • KIAA0253 antibody
    • Ncstn antibody
    • NCT antibody
    • NICA_HUMAN antibody
    • Nicastrin antibody
    • RP11 517F10.1 antibody
    • RP11517F101 antibody
    see all

Anti-Nicastrin antibody images

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Nicastrin with Anti-Nicastrin antibody (ab45425) at 20μg/ml.
  • Lane 1 : Anti-Nicastrin antibody (ab45425) at 0.5 µg/ml
    Lane 2 : Anti-Nicastrin antibody (ab45425) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    anti-rabbit HRP at 1/10000 dilution

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Additional bands at : 54 kDa,58 kDa,95 kDa. We are unsure as to the identity of these extra bands.
  • ab45425 at 2.5 µg/ml staining Nicastrin in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunofluorescence of Nicastrin in Human Brain cells using ab45425 at 20 ug/ml.

  • ab45425 staining Nicastrin in Human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2500 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

References for Anti-Nicastrin antibody (ab45425)

This product has been referenced in:
  • D'Souza Y  et al. Characterization of Aldh2 (-/-) mice as an age-related model of cognitive impairment and Alzheimer's disease. Mol Brain 8:27 (2015). WB, Dot blot ; Mouse . Read more (PubMed: 25910195) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast carcinoma)
Specification Breast carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%

Mr. Carl Hobbs

Verified customer

Submitted Feb 25 2013

Thank you for contacting us yesterday in regards to the Anti-Nicastrin antibody (ab45425) and Anti-PEN2 antibody (ab109619).

As discussed over the phone, I have requested further information regarding the immunogen used to raise the two antib...

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Thank you for your patience. I have received a reply from the originator of ab45425 (Nicastrin antibody). The immunogenic peptide sequence is proprietary information but they have performed a sequence alignment for you. Based on the alignment resu...

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