Anti-Nicastrin antibody - Carboxyterminal end (ab62508)


  • Product nameAnti-Nicastrin antibody - Carboxyterminal end
    See all Nicastrin primary antibodies
  • Description
    Rabbit polyclonal to Nicastrin - Carboxyterminal end
  • SpecificityThis antibody reacts with Nicastrin
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 17 amino acid synthetic peptide from near the carboxy terminus of human Nicastrin

  • Positive control
    • Mouse brain lysate and human brain tissue


Associated products


Our Abpromise guarantee covers the use of ab62508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.


  • FunctionEssential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the nicastrin family.
  • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anterior pharynx defective 2 antibody
    • APH 2 antibody
    • APH2 antibody
    • ATAG1874 antibody
    • KIAA0253 antibody
    • Ncstn antibody
    • NCT antibody
    • NICA_HUMAN antibody
    • Nicastrin antibody
    • RP11 517F10.1 antibody
    • RP11517F101 antibody
    see all

Anti-Nicastrin antibody - Carboxyterminal end images

  • Lane 1 : Anti-Nicastrin antibody - Carboxyterminal end (ab62508) at 0.5 µg/ml
    Lane 2 : Anti-Nicastrin antibody - Carboxyterminal end (ab62508) at 1 µg/ml
    Lane 3 : Anti-Nicastrin antibody - Carboxyterminal end (ab62508) at 2 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse brain lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
  • Immunofluorescence of Nicastrin in Human Brain cells using ab62508 at 20 ug/ml.

References for Anti-Nicastrin antibody - Carboxyterminal end (ab62508)

This product has been referenced in:
  • Griffiths HH  et al. Prion Protein Interacts with BACE1 Protein and Differentially Regulates Its Activity toward Wild Type and Swedish Mutant Amyloid Precursor Protein. J Biol Chem 286:33489-500 (2011). WB ; Human . Read more (PubMed: 21795680) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast carcinoma)
Specification Breast carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Feb 25 2013

Thank you for contacting us.

The ab62508 antibody has been tested in IHC-P and WB application only so it is fully guaranteed to be used in these applications; the images are present on the online datasheet. In case the antibody does not work...

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