Anti-Nicastrin antibody - Carboxyterminal end (ab62508)

Overview

  • Product nameAnti-Nicastrin antibody - Carboxyterminal end
    See all Nicastrin primary antibodies
  • Description
    Rabbit polyclonal to Nicastrin - Carboxyterminal end
  • SpecificityThis antibody reacts with Nicastrin
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 17 amino acid synthetic peptide from near the carboxy terminus of human Nicastrin

  • Positive control
    • Mouse brain lysate and human brain tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionEssential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the nicastrin family.
  • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anterior pharynx defective 2 antibody
    • APH 2 antibody
    • APH2 antibody
    • ATAG1874 antibody
    • KIAA0253 antibody
    • Ncstn antibody
    • NCT antibody
    • NICA_HUMAN antibody
    • Nicastrin antibody
    • RP11 517F10.1 antibody
    • RP11517F101 antibody
    see all

Anti-Nicastrin antibody - Carboxyterminal end images

  • Lane 1 : Anti-Nicastrin antibody - Carboxyterminal end (ab62508) at 0.5 µg/ml
    Lane 2 : Anti-Nicastrin antibody - Carboxyterminal end (ab62508) at 1 µg/ml
    Lane 3 : Anti-Nicastrin antibody - Carboxyterminal end (ab62508) at 2 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse brain lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 78 kDa
    Observed band size : 78 kDa
  • Immunofluorescence of Nicastrin in Human Brain cells using ab62508 at 20 ug/ml.

References for Anti-Nicastrin antibody - Carboxyterminal end (ab62508)

This product has been referenced in:
  • Griffiths HH  et al. Prion Protein Interacts with BACE1 Protein and Differentially Regulates Its Activity toward Wild Type and Swedish Mutant Amyloid Precursor Protein. J Biol Chem 286:33489-500 (2011). WB ; Human . Read more (PubMed: 21795680) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast carcinoma)
Specification Breast carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Feb 25 2013

Thank you for contacting us.

The ab62508 antibody has been tested in IHC-P and WB application only so it is fully guaranteed to be used in these applications; the images are present on the online datasheet. In case the antibody does not work...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"