Anti-Nicastrin antibody [EPR2575Y] (ab68145)

Overview

  • Product nameAnti-Nicastrin antibody [EPR2575Y]
    See all Nicastrin primary antibodies
  • Description
    Rabbit monoclonal [EPR2575Y] to Nicastrin
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C-terminus of human Nicastrin

  • Positive control
    • HeLa cell lysate, HL-60 cell lysate, Jurkat cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab68145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 100-120, 130-160 kDa (predicted molecular weight: 78 kDa).
IP 1/40.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionEssential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the nicastrin family.
    • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • Anterior pharynx defective 2 antibody
      • APH 2 antibody
      • APH2 antibody
      • ATAG1874 antibody
      • KIAA0253 antibody
      • Ncstn antibody
      • NCT antibody
      • NICA_HUMAN antibody
      • Nicastrin antibody
      • RP11 517F10.1 antibody
      • RP11517F101 antibody
      see all

    Anti-Nicastrin antibody [EPR2575Y] images

    • All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HL-60 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 78 kDa
      Additional bands at : 100-120 kDa (possible isoform),130-160 kDa (possible isoform).

    References for Anti-Nicastrin antibody [EPR2575Y] (ab68145)

    ab68145 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"